Basic Course in Flow Cytometry

Postgraduate course

Course description

Objectives and Content

Aim

Basic introduction to flow cytometry. The theoretical part of the course should enable the students to understand applications for flow cytometry. In addition, the student will have hands-on training in analyzing flow cytometry data during the practical data analysis part of the course which will be introduced in seminars by the course leaders.

Content

Theoretical part:

  • The course leaders will give a theoretical introduction to flow cytometry and some of the most common applications.
  • Practical part: Hands-on data analysis training for both conventional and spectral flow cytometry

Practical part:

  • Hands-on data analysis training for both conventional and spectral flow cytometry

Learning Outcomes

On completion of the course HUIMM307 the student is able to/has:

Knowledge

  • understanding for basic applications for flow cytometry
  • knowledge on which and how many different fluorochromes one can use on a given flow cytometer
  • to evaluate the quality of flow cytometry data

Skills

  • plan and perform basic experiments using flow cytometry
  • quality control and analysis of flow cytometry data
  • present flow cytometry data in a scientific way

Competence

  • understanding of basic flow cytometry
  • understanding as to why to use flow cytometry in a certain research question
  • interpret basic flow cytometry data
  • understanding as to why to use flow cytometry in a certain research question
  • interpret basic flow cytometry data

ECTS Credits

5

Level of Study

Master students, PhD candidates

Semester of Instruction

Spring

There is no direct registration in this course, but each person that would like to attend has to contact Brith Bergum as there is a limited number of places.

Place of Instruction

Core facility for flow cytometry, Department of Clinical Science, Laboratory building 5th floor

Required Previous Knowledge

Basic knowledge on apoptosis and cell cycle.

Experience with basic laboratory techniques such as pipetting and centrifugation.

Recommended Previous Knowledge

Biology or molecular biology on bachelor level.

Access to the Course

The course will only be given if at least 4 students register for the course, and is limited to 10 students.

Course-coordinator decides who can participate.

Teaching and learning methods

Mandatory 1 week, from 09.00-16.00 of lectures/seminars and hands on data analysis

Compulsory Assignments and Attendance

Compulsory participation in all experiments.

Forms of Assessment

Oral exam and presentation of results, graded by an internal and external sensor

Grading Scale

A-F

Assessment Semester

Spring

Reading List

Flow cytometry - A Basic Introduction,

Wiki version; Michael Ormerod.

Course Evaluation

After every course

Examination Support Material

None

Course Coordinator

Brith Bergum

Course Administrator